Screening Inhibitors of Methylerythritol Phosphate (MEP) Pathway Enzymes
Gaspari, Brittany M. 1993-
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With various antibiotics becoming illegitimate due to bacteria building up resistance to them, it becomes imperative to find new antibiotics to replace the ones that no longer work. In theory, this can be accomplished by inhibiting the IspF enzyme which can be found within the Methylerythritol phosphate (MEP) pathway. Four different cell cultures, one treated and containing no IPTG, one treated and containing IPTG, a control with IPTG, and a control lacking IPTG were found to have grown successfully at a steady pace over a period of 5 hours. This means that the overexpression of IspF within the cell cultures does not affect their ability to grow at a steady rate. An overexpression of IspF resulted in a large peak pertaining to CMP, whereas an unaltered sample lacked a defined peak. This should be useful in future work because it will be easier to see if potential inhibitors are successful.