PCR, its development & application to my research
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This semester I have been involved in ligation of L3, a gene in the La France virus, into the PgemT vector system as well as transfecting Ecoli with our ligated vector. This entire process involves creating a cDNA copy of the L3 RNA viral gene, amplification of the cDNA utilizing PCR, ligating it in to PgemT and then transforming the bacteria. These are the experimental procedures that I followed.