The effect of RNA2 mutation DR7 on recombination of Brome mosaic virus RNA3
Schnizlein, Matthew K., 1994--
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Brome mosaic virus (BMV) is a positive-sense, single-stranded RNA virus that infects plants throughout the world, including barley, tobacco, and wheat. Although this virus is not known for its ability to cause widespread crop damage, researchers frequently utilize BMV to understand more about other RNA viruses, such as human immunodeficiency virus. RNA2 of BMV’s tripartite genome contains a sequence that is thought to be responsible for regulating recombination of RNA3. Currently, there are no studies investigating this function in the Nicotiana benthamiana plant model. Thus, determining how the RNA-dependent RNA polymerase of RNA2 influences recombination in this model will increase understanding of this important mechanism of viral fitness and evolution. A mutation (DR7) was induced in RNA2 and cloned into Agrobacterium tumefaciens. Following agro-infiltration into N. benthamiana, total RNA was extracted and RT-PCR was used to amplify BMV RNA3. Although viral RNA could not be amplified, this project demonstrated the importance of plant age in the infection cycle of BMV.