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dc.contributor.authorStafstrom, Joel P.
dc.contributor.authorStaehelin, L. Andrew
dc.date.accessioned2013-02-25T15:37:34Z
dc.date.available2013-02-25T15:37:34Z
dc.date.issued1987
dc.identifier.citationStafstrom JP, Staehelin LA (1987). A second extensin-like hydroxyproline-rich glycoprotein from carrot cell walls. Plant Physiol. 84:820-825.en_US
dc.identifier.issn0032-0889
dc.identifier.urihttp://commons.lib.niu.edu/handle/10843/13478
dc.identifier.urihttp://hdl.handle.net/10843/13478
dc.description.abstractThe insoluble extensin matrix of dicot cell wails has been studied most fruitfully by examining the salt-extractable precursors to this matrix. Multiple extensin-like hydroxyproline-rich glycoproteins (HRGPs) have been isolated, or their existence inferred, from tomato, potato, bean, soybean, melon, carrot, and other plants. We and others previously have studied a carrot extensin which we call extensin-1. Here we report on the properties of extensin-2, a second salt-extractable hydroxyproline-rich glycoprotein from carrot. Like extensin-1, extensin-2 contains large amounts of hydroxyproline, serine, histidine, and lysine. In contrast, its tyrosine content is only about one-third that of extensin-l. Arabinose and alactose are the most abundant neutral sugars in both proteins, and nearly identical buoyant densities in CsCI suggest a similar proportion of carbohydrate in each. The size of extensin-2 is about half the size of extensin-1 based on: (a) the measured lengths of shadowed molecules (about 40 versus 84 nanometers); (b) the migrtion of extensin-2 in acidurea gels relative to monomers, dimers, and trimers of extensin-l; and (c) the Stokes' radii of these molecules as determined by gel filtration chromatography. Electron microscopy of shadowed extensin-2 molecules indicates that they contain kinks, which may indicate the presence of intramolecular isodityrosine cross-links, but intermolecular cross-links, either with other extensin-2 molecules or extensin-1 molecules, are observed rarely if ever.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society of Plant Biologistsen_US
dc.subjectHRGPen_US
dc.titleA Second Extensin-Like Hydroxyproline-Rich Glycoprotein from Carrot Cell Walls'en_US
dc.typeArticleen_US
dc.contributor.departmentDepartment of Biological Sciences


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