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Targeting an HIV-1 sequence in vivo by the hairpin ribozyme
(Northern Illinois University, 1992)
Ribozymes are small catalytic RNAs that can be engineered to cleave heterologous RNA in an enzymatic fashion. One of the applications of ribozyme technology is as an AIDS therapeutic. In this thesis, an HIV-1 sequence was ...
Elucidating the catalytic mechanism of the hairpin ribozyme
(Northern Illinois University, 1999)
The information reported here completes a mutational analysis of every base position located within the required loops of the hairpin ribozyme-substrate complex. In all, 24 base positions were analyzed using a point-mutational ...
In vivo repair of viral transcripts following cleavage by a cis hairpin ribozyme
(Northern Illinois University, 1996)
The hairpin ribozyme is a catalytic RNA that can be engineered to cleave any RNA with the sequence 5' GUC 3'. Because of this capacity to cleave targeted RNA, the hairpin ribozyme has the potential to down-regulate any ...
Trans-targeting of human papillomavirus type-16 E6 and E7 transcripts by an engineered hairpin ribozyme
(Northern Illinois University, 1995)
Three hairpin ribozymes have been designed which cleave sequences from HPV-16 E6 and E7 transcripts in vitro. E6 and E7 proteins from high risk HPV types are capable of immortalizing human cells and are therefore considered ...
A strategy for the determination of RNA autolysis in carrot protoplasts
(Northern Illinois University, 1990)
Over the past eight years, several examples of catalytic RNAs have been identified and shown to cleave. In this study, a self-cleaving RNA cassette, derived from the "hairpin" catalytic RNA, will be used to test for in ...
A hairpin ribozyme from a satellite RNA of chicory mottle virus : cleavage efficiency, substrate selectivity and targeting HIV1
(Northern Illinois University, 1996)
The catalytic capacity, substrate selectivity and application in HIV1 treatment of a hairpin ribozyme derived from sCYMVl were studied. Based on the sequence comparisons of hairpin ribozymes derived from sTRSV (GUC substrate) ...
Targeting tobacco mosaic virus with a hairpin ribozyme
(Northern Illinois University, 1992)
In vitro studies suggest that catalytic RNA may down regulate gene expression through cleavage of target RNA. This application is being evaluated by using a "hairpin" ribozyme derived from the negative strand of the satellite ...
A strategy for the determination of in vivo activity of a hairpin ribozyme
(Northern Illinois University, 1990)
Over the past ten years science has seen a gradual development of a new technology which involves the use of catalytic RNA molecules termed ribozymes. These unique forms of RNA have been shown to accurately and efficiently ...
The evaluation of a hairpin ribozyme to down regulate maize alcohol dehydrogenase expression in vivo
(Northern Illinois University, 1993)
Catalytic RNA is being used as a new tool to study the down regulation of gene expression. Our objective was to develop a model system to test the ability of the "hairpin" ribozyme to cleave target message RNA in vivo. A ...
In vitro characterization of two hairpin ribozymes that cleave HIV-1 RNA
(Northern Illinois University, 1993)
The hairpin ribozyme has been shown to cleave several heterologous RNA targets. In this thesis two hairpin ribozymes were shown in vitro to cleave two separate RNA sequences that are expressed by the virus in vivo. In vitro ...