Recombination of brome mosaic virus genomic RNA3 between fescue strain and Russian strain in barley protoplasts
Rao, Parth R.
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The study was performed to map the homologous recombination crossover regions between the genomic RNA3 of Fescue strain and Russian strain of Brome Mosaic Virus in barley protoplasts. Homologous recombination crossover regions between the genomic RNA 2 of both strains were analyzed previously in high selection pressure system using three different co-infected host plants. Thus, protoplast system was used to lower the selection pressure by eliminating the systemic infection phase. Both strains were introduced in different concentration to observe if the parental strain dominance can be lowered in the final clones. However, fescue strain showed dominance over Russian strain in two out of three ratios in BMV RNA3 co-inoculation. There were 12 crossover regions generated within RNA3, and most of them were consistent in all three ratios. Intercistronic region and PE regions were obvious hotspots observed in recombinants of all three ratios. Co-inoculation of RNA2 in protoplasts confirmed the hotspots mapped in the previous study in whole plant system. Also, analysis of RNA2 clones in protoplasts resulted in similar recombination frequencies to the whole plant systems experiment regardless of the selection pressure, which shows true mosaic nature of BMV.