Origin and development of protein bodies in seed cotyledons of Pharbitis nil : an ultrastructural and morphometric analysis
Leesman, Jeanne B.
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The development of protein bodies in seed cotyledons of Japanese morning glory (Pharbitis nil) was examined using transmission electron microscopy combined with morphometry. In cotyledonary tissue that was examined 24 days after anthesis, cells were highly vacuolate with no evidence of protein deposition. By 25 days post-anthesis, dictyosomes and rough endoplasmic reticulum cisternae were prominent features of the cytoplasm, and vacuoles contained proteinaceous deposits. As the cells matured, proteinaceous material occupied most of the vacuolar volume. In addition, small protein bodies were observed within the cytoplasm. By 44 days post-anthesis, deposition of storage reserves was more or less complete. Our observations were consistent with the suggestion of previous investigators, that protein bodies form as a consequence of an accumulation of proteinaceous material within the vacuoles as well as via dictyosome derived vesicles.