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dc.contributor.advisorRoston, Daryl A.en_US
dc.contributor.authorVer Vers, Leonard M.en_US
dc.date.accessioned2018-08-08T15:24:34Z
dc.date.available2018-08-08T15:24:34Z
dc.date.issued1984
dc.identifier.urihttps://commons.lib.niu.edu/handle/10843/18312
dc.descriptionBibliography : pages 55-56.en_US
dc.description.abstractA new detection system for the analysis of 6-thioguanine (6-TG) and 6-thioguanine riboside (6-TGR) in plasma samples has been investigated. The investigation began with a study of the electrochemistry of 6-TG and 6-TGR. First, cyclic voltammograms, were obtained for each compound. Second, hydrodynamic voltammograms were obtained. From these two voltammograms, the optimal working voltage of 1.00 volt vs. Ag/AgCl reference for oxidative thin-layer amperometry was noted. Another set of hydrodynamic voltammograms using a mercury electrode were obtained for 6-TG and 6-TGR. From these two voltammograms the detector potential of 0.20 volts vs. Ag/AgCl reference was noted as the working potential. After the optimal working potentials were obtained, linearity experiments were performed using three modes of detection: U.V. absorption, oxidative amperometry and mercury electrode detection of the sulfhydryl group. From a standard solution range of 1.0-200 ng, calibration curves for 6-TG and 6-TGR using all three modes of detection were obtained. Using the data from the calibration curves, regression analysis for both compounds was performed. Then using the Student's t-test at the 95% level, detection limits for both compounds using each detection mode were estimated. The U.V. absorption detection mode had the highest estimated detection limits: 0.91 ng for 6-TG and 0.94 ng for 6-TGR. The Au/Hg electrode had estimated detection limits of 49 pg for 6-TG and 73 pg for 6-TGR. Finally, oxidative amperometry produced estimated detection limits of 14 pg for 6-TG and 31 pg for 6-TGR. The two electrochemical detection modes produced estimated detection limits lower than any previously published. The feasibility of using electrochemical detection for the determination of 6-TG and 6-TGR in clinical samples was shown by comparing plasma chromatograms observed with U.V. absorption, oxidative electrochemical and Au/Hg detection.en_US
dc.format.extentviii, 56 pagesen_US
dc.language.isoengen_US
dc.publisherNorthern Illinois Universityen_US
dc.rightsNIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.en_US
dc.subject.lcshThioguanineen_US
dc.subject.lcshLiquid chromatographyen_US
dc.subject.lcshBlood--Analysisen_US
dc.subject.lcshElectrochemistryen_US
dc.titleEvaluation of liquid chromatography with electrochemical detection for determination of 6-thioguanine in clinical samplesen_US
dc.type.genreDissertation/Thesisen_US
dc.typeTexten_US
dc.contributor.departmentDepartment of Chemistryen_US
dc.description.degreeM.S. (Master of Science)en_US


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