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dc.contributor.advisorHampel, Arnold E.en_US
dc.contributor.authorTritz, Richarden_US
dc.date.accessioned2018-08-08T15:11:47Z
dc.date.available2018-08-08T15:11:47Z
dc.date.issued1985
dc.identifier.urihttps://commons.lib.niu.edu/handle/10843/18215
dc.descriptionBibliography: pages 39-44.en_US
dc.description.abstractLeucyl tRNA synthetase (LeuRS) has been purified several hundred-fold from Chinese hamster liver. The purification scheme was as follows: differential centrifugation, Alumina C gamma adsorption, DE-52 anion exchange column chromatography, and finally IGGF cation exchange column chromatography. The partially purified LueRS fraction obtained above was used as antigen in the production of monoclonal antibodies. Two immunization procedures were used. The in vivo procedure involved biweekly intraperitoneal injections of antigen in complete Freund's adjuvant. The in vitro procedure was done in 25 cm² culture flasks and the medium contained a synthetic peptidyl adjuvant. Using either procedure, the relative purity of the LeuRS fraction was important in the efficiency of specific anti- LeuRS clones produced.en_US
dc.format.extentvi, 44 pagesen_US
dc.language.isoengen_US
dc.publisherNorthern Illinois Universityen_US
dc.rightsNIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.en_US
dc.subject.lcshMonoclonal antibodiesen_US
dc.subject.lcshEnzymesen_US
dc.subject.lcshRibonucleic aciden_US
dc.titlePurification of Leucyl-tRNA synthetase and preparation of monoclonal antibodiesen_US
dc.type.genreDissertation/Thesisen_US
dc.typeTexten_US
dc.contributor.departmentDepartment of Biological Sciencesen_US
dc.description.degreeM.S. (Master of Science)en_US


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