Characterization of the pigment from Nocardia opaca
McClure, Melinda M.
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Nocardia opaca was grown under various environmental conditions and the pigments characterized. Two temperatures, 25C and 37C, were used with six different types of media: Brain Heart Infusion Agar (Difco), Plate Count Agar (Difco), Czapek's Dox Agar (Difco), Czapek's Dox Agar with 4.0% tryptone, Czapek's Dox Agar with 4.0% peptone, and Czapek's Dox Agar with 0.01% MnSC>4. The pigments were first analyzed for their visible spectra in a spectrophotometer and were then separated into their component fractions by thin layer chromatography. The amounts of these fractions obtained were inadequate to run further analyses. However, many similarities were noted among the pigments obtained as a result of medium and temperature variations. The most vivid color appeared on the most enriched medium, Brain Heart Infusion Agar, at 25C. This pigment also had the most fractions when analyzed with thin layer chromatography. Morphological differences were also noted when grown on the various media. Results show that the pigment could possibly be carotenoid in nature with certain side groups that cause shifts in maximum absorption. The solvents used for spectral analyses variously shifted the wavelength characteristics of the pigment adsorption spectra.