Characterization of the regulatory gene, mpkB, by overexpression studies in the model fungus, Aspergillus nidulans
Seif, Maie M.
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The main goal of this research was to understand the function of the mpkB gene (a mitogen activated protein kinases gene, MAPK) in the model fungus Aspergillus nidulans. Due to the similarity in DNA sequence with other homologous genes found in other fungi, such as Fus3 and Kss 1 genes in yeast, it is predicted that mpkB will also have a similar function. This research was conducted by transforming A. nidulans with a plasmid containing the mpkB gene attached to a strong inducible promoter, ale A. The plasmids also contain an auxotrophic marker for selection purposes, trpC. Results of this study will provide a better understanding of signal transduction pathways in filamentous fungi and how they affect processes such as mating in fungi. Since the genus Aspergillus includes species of great importance in medicine and agriculture, these results could also have a significant impact on these fields.