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dc.contributor.advisorJohnson-Wint, Barbaraen_US
dc.contributor.authorGwillim, Eran C.en_US
dc.date.accessioned2016-10-24T17:53:47Z
dc.date.available2016-10-24T17:53:47Z
dc.date.issued2009
dc.identifier.urihttp://commons.lib.niu.edu/handle/10843/16778
dc.descriptionIncludes bibliographical references.en_US
dc.description.abstractIn preparation to quantify beta-actin and alpha-smooth muscle actin content in loaded and unloaded native rat tail tendon organ culture models, several methodologies were developed in the present paper. Since alpha-smooth muscle actin has not been monitored in tendon in vitro, developing the methods to culture and quantitatively detect alpha-smooth muscle actin, the protein marker for fibroblast to myofibroblast transition were developed. In addition, quantifying the amount of alpha-smooth muscle actin mRNA, using reverse transcription polymerase chain reaction (RT-PCR) assays was investigated and novel oligonucleotide primers were developed. A method to identify four highly conserved actin isoforms; beta cytoplasmic, gamma cytoplasmic, alpha- smooth muscle and gamma smooth muscle actin, in a tissue environment was created.en_US
dc.format.extent23 pagesen_US
dc.language.isoen_USen_US
dc.publisherNorthern Illinois Universityen_US
dc.rightsNIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.en_US
dc.subjectconnective tissue repairen_US
dc.subjectActin Isoformsen_US
dc.titleDense connective tissue repair : the intricacies of monitoring actin isoforms in native tissue models in vitroen_US
dc.type.genreDissertation/Thesisen_US
dc.typeTexten_US
dc.contributor.departmentDepartment of Biological Sciencesen_US
dc.description.degreeB.S. (Bachelor of Science)en_US


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