Morphometric analysis of mycorrhizal fungus in Allium cepa L. roots
Bennett, Elizabeth Lee
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Allium cepa L. seeds were planted in soil inoculated with Glomus fasciculatum and grown for a period of 90 days in the greenhouse. Sectioned roots were examined with a light microscope and fungal structures were counted using a Weibel reticle in the ocular of the microscope. The absolute volume of the root occupied by developing arbuscules, degenerating arbuscules, intercellular hyphae, intracellular hyphae and vesicles was determined. It was found that 4% of the root-volume was occupied by the fungus, of which 0.78% was occupied by developing arbuscules, 0.58% by degenerating arbuscules, 2.35% by intercellular hyphae, 0.02% by intracellular hyphae and 0.28% by vesicles. By day 90, the volume of the root occupied by developing arbuscules, degenerating arbuscules and intercellular hyphae had increased significantly while the volume occupied by intracellular hyphae had decreased. The volume occupied by vesicles was variable over the 90 days but appeared to be increasing by day 90.