The substrate specificity of Bacillus cereus Phospholipase C
Mueller, Sandra M.
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The purpose of this thesis is to investigate the substrate specificity of Bacillus cereus Phospholipase C. The following three questions more specifically outline the primary objective of the study. I Does the enzyme prefer monomer substrates with long or short carbon chains? II Does the enzyme prefer substrates which form micelles or vesicles? Ill Does the enzyme prefer monomer or aggregate substrates? These three questions are answered on the basis of the experimental results obtained. A series of phosphatidylcholine thiol analogs with varying carbon chain length were synthesized for the specific purpose of the study. Physical properties, such as critical micelle concentration and ultrastructure formation were determined for each substrate in the series, and enzyme activity on both the monomer and aggregate form of each substrate was observed.