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dc.contributor.advisorHudspeth, Michael E. S.en_US
dc.contributor.authorSachay, Donna Jeanen_US
dc.date.accessioned2016-03-04T20:00:01Z
dc.date.available2016-03-04T20:00:01Z
dc.date.issued1989
dc.identifier.urihttp://commons.lib.niu.edu/handle/10843/15914
dc.descriptionIncludes bibliographical references (pages [62]-69)en_US
dc.description.abstractPrevious characterization of P. megasperma mitochondrial DNAs revealed two genic inversions between a host-specific and a nonspecific strain. As part of a characterization of oomycete mitochondrial genomes, studies of the nucleotide sequence of the region involving the COI-COII inversion were initiated. The COII genic region was localized, cloned and sequenced. The COII gene encodes a 258 amino acid protein with an approximate molecular weight of 29.6Kd. The data strongly suggests a lack of introns in this gene. The COI protein has been found to be encoded 235bp downstream from COII on the same strand. The translated COII amino acid sequence exhibits a greater than 50$ amino acid homology with other characterized eukaryotic COII proteins, being most homologous with those of plants. The two functionally conserved membrane-spanning helices and the putative copper-binding site, along with other conserved regions, have been identified in the protein. The genic region has an A-T content of 71$. Flanking regions contain 79$ and 90$ A-T contents. As expected for A-T-rich genomes, codon usage tables indicate a strong bias for the use of a third position A/T when choices exist. Of all codons within this gene, 85$ terminate in an A or T. Remarkably, however, all 7 Trp codons are TGG instead of the preferred TGA typical of all but plant mitochondria using a modified genetic code. This would lead to the conclusion that in Phytophthora mitochondrial DNA, TGA is either a stop codon, as it is in plant mitochondria, or an infrequently used trp codon.en_US
dc.format.extent[vii], 69 pagesen_US
dc.language.isoengen_US
dc.publisherNorthern Illinois Universityen_US
dc.rightsNIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.en_US
dc.subject.lcshCytochrome oxidaseen_US
dc.subject.lcshCytogeneticsen_US
dc.subject.lcshDNAen_US
dc.titleThe absence of TGA codons in the DNA sequence of the Phytophthora megasperma gene for cytochrome c oxidase subunit IIen_US
dc.type.genreDissertation/Thesisen_US
dc.typeTexten_US
dc.contributor.departmentDepartment of Biological Sciencesen_US
dc.description.degreeM.S. (Master of Science)en_US


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