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dc.contributor.advisorHampel, Arnold E.en_US
dc.contributor.authorHoenle, Robert J.en_US
dc.date.accessioned2016-02-26T17:27:32Z
dc.date.available2016-02-26T17:27:32Z
dc.date.issued1990
dc.identifier.urihttp://commons.lib.niu.edu/handle/10843/15694
dc.descriptionIncludes bibliographical references (pages [61]-67)en_US
dc.description.abstractOver the past ten years science has seen a gradual development of a new technology which involves the use of catalytic RNA molecules termed ribozymes. These unique forms of RNA have been shown to accurately and efficiently cleave heterologous RNAs in vitro and evidence is now emerging that may show that ribozymes can function in an in vivo enviroment. The purpose of this study is to present evidence of in vivo ribozyme activity on the phenotypic and molecular level. A ribozyme of the hairpin type was incorporated into the genome of Chinese hamster ovary cells along with its target, the chloramphenicol acetyl transferase gene, via calcium phosphate precipitation. A sensitive assay was developed using the endonuclease Si and radioactive probes to search for the presence of the ribozyme, the presence of CAT mRNA and the expected cleavage products. The outcome of the experiment was hindered by the unexpected instability of CAT mRNA driven by the promoter used in this study. The stable existence of the ribozyme however was confirmed.en_US
dc.format.extent67 pagesen_US
dc.language.isoengen_US
dc.publisherNorthern Illinois Universityen_US
dc.rightsNIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.en_US
dc.subject.lcshCatalytic RNAen_US
dc.titleA strategy for the determination of in vivo activity of a hairpin ribozymeen_US
dc.type.genreDissertation/Thesisen_US
dc.typeTexten_US
dc.contributor.departmentDepartment of Biological Sciencesen_US
dc.description.degreeM.S. (Master of Science)en_US


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