Show simple item record

dc.contributor.authorGuss, Cheryl A.en_US
dc.date.accessioned2016-02-05T14:48:38Z
dc.date.available2016-02-05T14:48:38Z
dc.date.issued1993
dc.identifier.urihttp://commons.lib.niu.edu/handle/10843/15290
dc.descriptionIncludes bibliographical references (pages [58]-61)en_US
dc.description.abstractCatalytic RNA is being used as a new tool to study the down regulation of gene expression. Our objective was to develop a model system to test the ability of the "hairpin" ribozyme to cleave target message RNA in vivo. A synthetic hairpin ribozyme was designed to cleave the maize alcohol dehydrogenase 1 (Adh1) message. Adh1 was chosen because activity of the protein is easily assayable and a selection system for stable transformants is available. The synthetic ribozyme (RADH) was designed to target native Adh1 mRNA at nucleotide position 3093 containing the requisite GUC cleavage site. Two pUC19 based vectors were engineered for in vivo expression of RADH at high levels in maize protoplasts. Transient and stable expression assays were performed to evaluate cleavage of the Adh1 mRNA transcript.en_US
dc.format.extentvi, 63 pagesen_US
dc.language.isoengen_US
dc.publisherNorthern Illinois Universityen_US
dc.rightsNIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.en_US
dc.subject.lcshCatalytic RNAen_US
dc.subject.lcshGenetic regulationen_US
dc.subject.lcshGene expressionen_US
dc.titleThe evaluation of a hairpin ribozyme to down regulate maize alcohol dehydrogenase expression in vivoen_US
dc.type.genreDissertation/Thesisen_US
dc.typeTexten_US
dc.contributor.departmentDepartment of Biological Sciencesen_US
dc.description.degreeM.S. (Master of Science)en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record