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dc.contributor.authorMorgan, Aricen_US
dc.date.accessioned2015-11-30T21:38:12Z
dc.date.available2015-11-30T21:38:12Z
dc.date.issued2000
dc.identifier.urihttp://commons.lib.niu.edu/handle/10843/14441
dc.descriptionIncludes bibliographical references (pages [75]-76)en_US
dc.description.abstractThis thesis characterizes the reaction between K2IrCl6 and ZnCcP. This reaction was compared to previously reported data of the reaction between K2IrCl6 and two other heme proteins, metmyoglobin (metmb) and horseradish peroxidase (HRP). The reactions were monitored spectrophotometrically using diode array and stopped flow instruments. To characterize the reaction between ZnCcP and K2IrCl6, the objectives were to (1) generate the spectrum of the ZnCcP porphyrin cation radical, (2) to generate the equilibrium constant for the reaction, and (3) to generate the electron transfer rate constants. The results of the experiments showed that ZnCcP behaves differently than metmb or HRP. Previously reported data show that the reaction between K2IrCl6 and HRP and metmb have few side reactions. The reaction between ZnCcP and K2IrCl6 has a large number of side reactions. Also, the concentration of K2IrCl6 goes to zero indicating that there is no equilibrium between ZnCcP and K2IrCl6. The values for the electron transfer rate constants are about 2 s'-1 and 0.3 s'-1. The spectral data suggest that oxidation of the porphyrin ring occurs through oxidation of surface tryptophan residues.en_US
dc.format.extentix, 101 pagesen_US
dc.language.isoengen_US
dc.publisherNorthern Illinois Universityen_US
dc.rightsNIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.en_US
dc.subject.lcshPeroxidaseen_US
dc.subject.lcshCytochrome cen_US
dc.subject.lcshCytochrome oxidaseen_US
dc.subject.lcshPotassium compoundsen_US
dc.subject.lcshChlorine compoundsen_US
dc.titleCharacterization of the reaction between cytochrome c peroxidase and potassium hexachloroiridateen_US
dc.type.genreDissertation/Thesisen_US
dc.typeTexten_US
dc.contributor.departmentDepartment of Chemistry and Biochemistryen_US
dc.description.degreeM.S. (Master of Science)en_US


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