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Effect of oncogenic virus on muscle differentiation

Show simple item record Holtzer, H. en_US Biehl, J. en_US Yeoh, J. en_US Meganathan, R. en_US Kaji, A. en_US 2015-06-10T16:34:01Z 2015-06-10T16:34:01Z 1975-10
dc.identifier.citation Holtzer, H., J. Biehl, G. Yeoh, R. Meganathan and A. Kaji. 1975. Effect of oncogenic virus on muscle differentiation. Proc. Nat. Acad. Sci. USA, 72:4051-4055. en_US
dc.description.abstract Chick muscle cultures infected with wild-type Rous sarcoma virus form myotubes, but these myotubes vacuolate and by day 6 most have degenerated, leaving only large numbers of transformed mononucleated, replicating cells. Muscle cultures infected with a temperature-sensitive mutant (TS) at permissive temperatures behave as cells infected with wild-type Rous sarcoma virus. TS-infected cells reared for 8 days at nonpermissive temperature form contracting myotubes, plus large numbers of fibroblastic cells. If these cultures are lowered to permissive temperature, within 72 hr the myotubes vacuolate and degenerate, whereas the mononucleated cells transform. If replicating TS-transformed cells after 8 days at permissive temperature are shifted to nonpermissive temperature, within 72 hr many cells fuse and form contracting, post-mitotic myotubes. Creatine kinase (ATP:creatine N-phosphotransferase, EC levels parallel the formation and degeneration of myotubes during these temperature shifts. If the viral transforming gene is expressed in the post-mitotic myotubes it is lethal, whereas it is not lethal if expressed in replicating precursor myogenic cells. The viral gene expression at permissive temperature blocks further myogenesis depending on the position of the cells in the myogenic program. The virus does not cancel the replicating, transformed myogenic cells' commitment to, or position in, the myogenic lineage. When the transforming action of the virus is suppressed, the normal myogenic program resumes. en_US
dc.format.extent 5 pages en_US
dc.language.iso en_US en_US
dc.publisher Proceedings of the National Academy of Sciences of the United States of America en_US
dc.subject myogenesis en_US
dc.subject temperature-sensitive Rous sarcoma virus en_US
dc.subject transformed myogenic cells en_US
dc.subject creatine kinase en_US
dc.title Effect of oncogenic virus on muscle differentiation en_US
dc.type.genre Article en_US
dc.type Text en_US
dc.contributor.department Department of Biological Sciences

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